Composite

Part:BBa_K2969045:Design

Designed by: Liangchen Zhuo   Group: iGEM19_UCAS-China   (2019-10-16)


pTetO(wt)-mfLon


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2464
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None


Source

Zheng, Y., Meng, F., Zhu, Z., Wei, W., Sun, Z., Chen, J., . . . Chen, G.-Q. (2019). A tight cold-inducible switch built by coupling thermosensitive transcriptional and proteolytic regulatory parts. Nucleic Acids Research. doi:10.1093/nar/gkz785 In this paper, the author combined the thermosensitive proteolytic with transcriptional regulatory parts to build a tight cold-inducible switch and put it into various applications

References